Agarose Particles for Purification of Protein

Published by: richardjgray, Thu Mar 14 2024

Agarose Particles

With years of experience in the pharmaceutical and life science sectors, CD Bioparticles proudly announces the launch of its latest product line of Agarose Particles for isolation and purification of proteins and antibodies. These versatile microspheres, available in diameters ranging from 25 nm to 500 µm, empower researchers with a powerful tool for a wide range of applications. Agarose particles are porous gel microspheres that, depending on their size, can filter or separate mixtures of molecules. These particles are easily activated to bind reversibly or irreversibly to biomolecules. They can be used for batch or column purification of proteins and antibodies and are well suited for biopharmaceutical applications. In addition, their large internal porosity allows the purification of larger biomolecules such as monoclonal antibodies. Meanwhile, their particle size distribution is mainly between 20 and 300 μm. Small particle sizes are suitable for very high resolution polishing steps, while large particle sizes are suitable for capture steps. Furthermore, the volume required also influences the choice of particle size, as large volumes typically require larger particle sizes. Agarose particles are available in different percentages, e.g., 2%, 4% and 6%, allowing better separation of molecules according to their size. Particles with a higher percentage of agarose are suitable for separating small globular proteins due to their smaller pore size. Similarly, particles with a lower percentage of agarose are more suitable for separating larger globular proteins. In addition, preactivated agarose particles can be used to bind biomolecules. Particles with proteins and antibodies on the surface are used as affinity chromatography resins, ion exchange chromatography resins, size-exclusion chromatography resins, and hydrophobic interaction chromatography resins. Enzyme-immobilized agarose particles can be used for antibody fragmentation.

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